Antileishmanial Agents: A Review

 

Parmesh Kumar Dwivedi1, Kishu Tripathi2, Mamta Mishra3 and Suprabhat Ray1

1Amity Institute of Pharmacy, Amity University, Uttar Pradesh, Lucknow Campus, Lucknow

2Institute of Pharmaceutical Sciences and Research Center, Bhagwant University, Ajmer. Rajasthan

3State Public Analytical Laboratory (FDA), Lucknow

 

 

ABSTRACT:

Leishmaniasis is a disease caused by number of protozoa in the genus leishmania,is a complex of diseases caused by 17 species of the protozoan parasite Leishmania and transmitted by~30 species of phlebotomine sandflies. Current treatment strategies and problems associated with it are given.

 

KEYWORDS:  Leishmaniasis, antileishmanial

 

INTRODUCTION:

Leishmaniasis is a disease caused by a number of protozoa in the genus leishmania. The protozoa may be harbored in diseased rodents, canines and various other mammals, transmitted from the infected mammal to man by bites from female sandflies of the genus phlehotomus and then appear in one of the four major clinical syndromes: visceral leishmaniasis, cutaneous leishmaniasis, mucocutaneous leishmaniasis or diffused cutaneous leishmaniasis. As many as twelve million individuals, worldwide are infected by this organism. The visceral leishmaniasis, also known as kala azar (black fever), is caused by Leishmania donovani1. Leishmaniasis, a vector born-disease that is caused by obligate intra-macrophage protozoa, is endemic in large areas of the tropics, subtropics and Mediterranean basin1.  

 

Leishmaniases are a complex of diseases caused by 17 species of the protozoan parasite Leishmania and transmitted by~30 species of Phlebotomine sandflies.

 

 

Current treatment strategies and problems associated with it:

Treatment of leishmaniasis relies on specific antileishmanial drugs, and in case of visceral leishmaniasis(VL) also an aggressive management of concomitant bacterial or parasitic infection, anemia, hypovolemial (decreased blood volume), and malnutrition, is needed. The drugs used for above purpose are described below:

 

A) Pentavalent antimonials:

Pentavalent antimonials sodium stibogluconate(60) and meglumine antimoniate (59) have been the first line treatment in leishmaniasis for more than 70 years. Structure of sodium stibogluconate is still not known. The drug gets activated within amastigote but not in the promastigote, by conversion to the lethal trivalent form, the mechanism of which is not known. It exerts its antileishmanial effect might be due to the action on host macrophages2.

 


 

 

Antimonials are toxic drugs with frequent sometimes life threatening (in case of VL), adverse side effects, including cardiac arrhythmia and acute pancreatitis. Due to its toxicity and requirement of 28 days of parenteral administration (patient’s compliance) and emergence of significance resistance has restricted its use3.

 

B) Pentamidine:

The usefulness of diamidine pentamine (61) as antileishmanial drug has been limited by its toxicity. Nevertheless since its introduction in 1952, it has had value for cutaneous leishmaniasis (CL), VL and diffuse CL. It is normally used as a second line drugs, when antimonials have proved ineffective.4

 

C) Amphotericin B:

The polyene antibiotics Amphotericin B(62) has proved to be highly effective for the treatment of antimonial resistant L. donovani VL and cases of mucocutaneous leishmaniasis (MCL), that have not responded to antimonials but it is a unpleasant drug because of its toxicity and the need for slow infusion of parenteral administration over 4 hours5.

 

D) Miltefosine:

Miltefosine (63), an alkyl phosphocholine which was initially developed as an anticancer drug, is the first effective oral drug for VL. In a recent phase IV trial, final cure rate was 82 % by intention to treat with only 3 deaths out of 1132 patients6.

 

D) Miltefosine:

Miltefosine (63), an alkyl phosphocholine which was initially developed as an anticancer drug, is the first effective oral drug for VL. In a recent phase IV trial, final cure rate was 82 % by intention to treat with only 3 deaths out of 1132 patients6.

 

E) Paromomycin:

Paromomycin (64), is an aminoglycoside antibiotic with good antileishmanial activity7. The result of phase 3 trials recently conducted in India showed excellent efficacy and safety. No nephrotoxicity was observed, reversible high tone ototoxicity (damage to inner ear) was found in 2% of patients and 1.8% patients showed significant increase (> 5 fold) in hepatic transaminases.8

 

Antileishmanial activity of sitamaquine (65) was first identified in 1970 at the Walter Reed Army Institute of Research. Phase 2 study was conducted in Brazil, Kenya and India and obtained cure rates ranging from 27-87 %, but there were also several cases of severe renal adverse events.9

 

 

G) Combination Therapy:

It is suggested that the way forward is to increase treatment efficacy, prevent development of drug resistance, reduce treatment duration and decrease treatment cost.

 

The association of sodium stibogluconate and paramomycin was found to be safe and effective in early trials conducted in India and East Africa10. The drug combination including liposomal amphotericin B and miltefosine are currently being studied in India.

 

REFERENCES:

1.        Clive R.; Paul K.; Simon L C.; Shyam S., B.M.J. 2003,326, 377–82.

2.        Pathak, M.K.; and Yi, T., J.Immunol. 2001, 167, 3391–3397.

3.        Sundar, S.,  Clin. Infect. Dis. 2000, 31, 1104–1107.

4.        Wilson, W. D.; Nguyen, B.; Tanious, F. A.; Mathis, A.; Hall, J. E.; Stephens,C. E.; Boykin, D. W,. Curr. Med. Chem. Anti-Cancer Agents 2005, 5, 389-408..

5.        Roberts, C.W. , Mol. Biochem. Parasitol. 2003, 126, 129–142.

6.        Berman, J. ,ExpertOpin. Pharmacother. 2005, 6, 1381-1388.

7.        Den B.; M. & Davidson, R. N,. Expert Rev. Anti Infect. Ther. 2006, 4, 187–197.

8.        Sundar, S., N. Engl. J. Med. 2007, 356, 2571–2581.

9.        Jha, T. K. , Am. J. Trop. Med. Hyg. 2005, 73, 1005–1011.

10.     Thakur, C. P., Trans. R. Soc. Trop. Med. Hyg. 2000, 94, 429–431.

 

 

Received on 14.12.2009

Accepted on 17.03.2010        

© A&V Publication all right reserved

Research Journal of Pharmacognosy  and Phytochemistry. 2(4): July-Aug. 2010, 264-266